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Because other notations have been used to express kinetic equations,
particulary for bireactant mechanisms, a partial comparison is shown for
sequential bireactant mechanism in table between system described in Cleland
notation and those often seen in the literature (see comments below).
NOMENCLATURE COMPARISON FOR SEQUENTIAL BI BI MECHANISMS
If the reaction order is obligatory, substrate A adds first.
Comments.
A steady-state velocity equation for Ordered Bi Bi enzyme reaction mechanism
in the absence of products (P=0, Q=0) can be written as:
, (1)
where A is first substrate, B - second substrate, Et - total enzyme concentration
and f1,...,f5 are different groups of rate constants.
Numerator and denominator of equation (1) are divided by f5 and the equation in new terms is obtained:
or, in reciprocal form:
,
where
.
Numerator and denominator of equation (1) are divided by f1 and the equation in new terms is obtained:
or, in reciprocal form:
,
where two substrate are indicated as S1 and S2, and
.
Numerator and denominator of equation (1) are divided by f2 and the equation in new terms is obtained:
,
or, in reciprocal form:
,
where
.
Numerator and denominator of equation (1) are divided by f5 and the equation in new terms is obtained:
or, in reciprocal form:
,
where
.
References:
Alberty R. A., (1953), J. Am. Chem. Soc., v.75, p.1928
Dalziel K., (1957), Acta Chem. Scan., v.11, p.1706
Bloomfield V., Peller L., Alberty R. A., (1962), J. Am. Chem. Soc., v.84, p.4367
Cleland W. W., (1963), Biochim. Biophys. Acta, v.67, pp.104, 173, 188.
[ERM Main Menu]
[About the ERM]
[Cleland notation]
[Enzyme kinetics]
[Alternate nomenclature]
[Publication]
Russian Academy of Science
Institute of Theoretical and Experimental Biophysics
Laboratory of Metabolic Simulation and Bioinformatics
Pushchino, Moscow reg., Russia, 142290.
Created by Milya Galimova.